Her first major film role was in 2005's The Sisterhood of the Traveling Pants, co-starring Alexis Bledel, America Ferrera, and Blake Lively.
She also starred in its 2008 sequel, The Sisterhood of the Traveling Pants 2.
In 2007, Tamblyn starred in the CBS pilot Babylon Fields, an apocalyptic comedic drama about the undead trying to resume their former lives. In the same year, Tamblyn had a recurring role alongside her then boyfriend (later husband) David Cross in the IFC sitcom The Increasingly Poor Decisions of Todd Margaret.
From November 2010 until April 2011, Tamblyn starred as medical student Martha M.
Masters, during the seventh season of the Fox medical drama series House. In August 2013, Tamblyn was cast as Charlie Harper's long-lost (and previously unknown) lesbian daughter, Jenny, on the long-running sitcom Two and a Half Men, opposite Ashton Kutcher and Jon Cryer.
Her first appearance was on the season 11 opener, September 26, 2013.
Tamblyn's father made several appearances as God in the form of a dog walker on the show, which ran from 2003 to 2005.
Early guest starring roles include: Buffy the Vampire Slayer (playing Janice Penshaw, the best friend of Dawn Summers), on Boston Public, CSI: Miami, and Punk'd (where Ashton Kutcher and his crew members tricked her into losing someone else's dog).
Amber Rose Tamblyn (born May 14, 1983) is an American actress, author, poet, and film director.
She first came to national attention in her role on the soap opera General Hospital as Emily Quartermaine, followed by a starring role on the prime-time series Joan of Arcadia, portraying the title character.